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KMID : 0613820100200111667
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Journal of Life Science
2010 Volume.20 No. 11 p.1667 ~ p.1674
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Influence of Nitrate on Growth, Chlorophyll Content, Content and Activity of Rubisco and Rubisco Activase of Tobacco Plant Treated with Cadmium in vitro
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Roh Kwang-Soo
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Abstract
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Influence of nitrate on growth, chlorophyll content, content and activity of rubisco and rubisco activase of tobacco plant cultured on MS medium treated with cadmium in vitro was studied. In vitro growth and chlorophyll content reduced at 0.2 mM Cd was recovered by nitrate and this recovery was most significant at 80 mM nitrate. Rubisco content at 80 mM nitrate was more increased compared to that at other concentrations. A similar change was also shown in rubisco activity. These results indicate that the activation and induction of rubisco reduced by Cd were recovered by nitrate. The degree of intensity of 55 and 15 kD polypeptides identified as the large and small subunits of rubisco by SDS-PAGE analysis at 80 mM nitrate was significantly higher than that at other concentrations. The content and activity of rubisco activase at 80 mM nitrate was significantly increased than that at other concentrations. These data suggest that the recovery effects of rubisco by nitrate may be associated with rubisco activase.
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KEYWORD
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Cd, chlorophyll, nitrate, rubisco, rubisco activase
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